Changes in both larval and adult zebrafish behavior can be linked to numerous phenotypes associated with addiction that include conditioned place preference for drugs of abuse, relapse, changes in social behavior, along with symptoms indicating the development of tolerance and withdrawal 90, 93, 96, 97, 98, 99. In our laboratory we are trying to develop novel learning and memory paradigms for zebrafish. Reward can been measured in zebrafish by using the conditioned place preference cpp test, which pairs a primary cue e. Zebrafish models in neurobehavioral research book, 2011. Various aspects of the animals behaviour can be measured with respect to the alternative environments, such as latency and frequency of entry, duration of time spent, range of activities observed, or relative consumption of a goal object in the environment. Gene expression changes in a zebrafish model of drug dependency. Overview studying fish behavior is both a fascinating and very practical endeavor for the fish keeper. Although a number of different designs and apparatuses are used in this model, the basic characteristics of this task involve the association of a particular environment with drug treatment, followed by the association of a different environment with. Conditioned place preference the conditioned place preference test is a great example of a zebrafish behavioral assay that has a clear analog in the world of rodent behavioral tests.
Initial experiments using only wildtype fish demonstrated a consistent and robust cocaineinduced cpp. Adult zebrafish as a model organism for behavioural genetics. In general, this task involves the establishment of an association between a specific environmental stimulus and a positive or a negative. Druginduced place conditioning is commonly used in mice to study the rewarding and aversive effects of drugs. The conditioned place preference procedure is classically used for a long time to test the addictive liability of putative drugs of abuse in research as well as in pharmaceutical industry. Zebrafish ecology and behaviour rowena spence olfactory behavior.
May 01, 2014 read use of conditioned place preferenceavoidance tests to assess affective states in fish, applied animal behaviour science on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. The zebrafish danio rerio, a prominent vertebrate model organ ism for developmental studies 2, is increasingly used for molecular genetic dissection of neural circuits and behavior 3,4. Videoaided analysis of zebrafish locomotion and anxietyrelated behavioral responses videograms. Caffeine did not have a significant effect on place preference at 50 mgl, 100 mgl or 150 mgl. Zebrafish were acutely exposed to ethanol and caffeine in the conditioned place preference assay. Modified associative learning tmaze test for zebrafish danio rerio and other small teleost fish georgianna g. Kedikian x, faillace mp, bernabeu r 20 behavioral and molecular analysis of nicotineconditioned place preference in zebrafish. Review open access adult zebrafish as a model organism for. Our lab has previously reported the development of a reliable conditioned place preference paradigm for zebrafish. All products is defined on a po or list price for nonregistered users. Pdf conditioned place preference behavior in zebrafish. Conditioned place preference behavior in zebrafish nature. Children who behave keep their fish out of the sea grass.
If animals develop an increase in preference to spend time in an environment that had previously been paired with drug administration, the drug is inferred to have rewarding properties. Aug 01, 2015 behavioral quantification within the conditioned place preference cpp paradigm serves as a measure of the rewarding qualities of a given stimulus e. The procedure may also be modified to determine whether genetically modified animals are more or less sensitive to the reinforcing effects of a drug. A guide to testing zebrafish behavior maze engineers. Conditioned place preference behavior in zebrafish article pdf available in nature protocol 63. Conditioned place preference cpp, where drug exposure is paired with. Behavioral and molecular analysis of nicotineconditioned. Zebrafish as a highthroughput in vivo model for testing the. Conditioned place preference cpp, which likely engages mechanisms similar to those of classical conditioning, has been widely used to determine the reinforcing effect of naturally rewarding e. Conditioned place preference conditioned recting place aversion hunger welfare motivation a b s t r a c t conditioned place preference cpp or aversion cpa methods are potentially useful tools in animal welfare assessment because they permit measurement of the reinforcing properties of a stimulus in the absence of the stimulus itself. Conditioned place preference methods of behavior analysis. Dissociation of food and opiate preference by a genetic mutation in. Once concentration and time of exposure to the drug were fixed, we characterized some specific responses for identifying preferencerelated behaviors to nicotineconditioning in zebrafish.
Apr 07, 20 psychology definition of conditioned place preference cpp. Assessing the value of the zebrafish conditioned place preference. Sep 30, 2016 this method provided further evidence that zebrafish actually develop a preference for nicotine, although the drug was administrated in an aversive place for the fish. Zebrafish behavior, social interaction, zebrafish shaoling home. Conditioned place preference cpp is a form of pavlovian conditioning used to measure the motivational effects of objects or experiences. Use of conditioned place preferenceavoidance tests to.
To view our sam, or fbo credentials please contact us for our cage code and duns number. Conditioned place preference for 8 days, lizards were allowed to move between two different chambers. Assessing the value of the zebrafish conditioned place preference model for predicting human abuse potential. Springer nature is developing a new tool to find and evaluate protocols. The conditioned place preference paradigm is a standard preclinical behavioral model used to study the rewarding and aversive effects of drugs.
Drug dependency can also be evaluated by measuring the persistence of cpp following a period of abstinence. Effects of dizocilpine mk801 on circling behavior, swimming activity, and place preference in zebrafish danio rerio. Conditioned place preference cpp, pavlovian conditioning, drug reward, drug selfadministration, drug abuse. The conditioned place preference test provides an evaluation of the effects of drugs in animal models, allowing the investigation of substances at a biologically relevant level with respect to reward. Conditioned place preference cpp, which likely engages mecha. Behavioral and molecular analysis of nicotineconditioned place preference in zebrafish article pdf available in plos one 87. The place preference box is a standard experimental chamber for automated assessment of conditioned place preference and aversion in rodents, two tests widely used for screening the reinforcing properties of drugs or natural stimuli as well as for investigating the brain neurobiological systems implicated in reward and addiction. The lowerright illustration 4 depicts an aquatic version of the place preference procedure used to measure conditioned appetitive stimuli. Zebrafish conditioned place preference cpp is a widely used paradigm to. Nicotineinduced place preference in zebrafish was corroborated by behavioral analysis of several indicators of drug preference, such as time spent in the drugpaired side, number of entries to the drug paired side, and distance traveled. This claim is supported by conditioned place preference behavior reported in zebrafish following administration of a wide range psychoactive substances that mirror mammalian cpp findings braida et al. Zebrafish conditioned place preference models of drug reinforcement and relapse to drug seeking amit parmar, miral parmar, and caroline h. A method for repeatable unbiased quantitative behavioral analysis without scoring or tracking automated imaging of avoidance behavior in larval zebrafish. In this test, a subject is first exposed to an unconditioned stimulus, e.
Here, we establish a reliable conditioned place preference. Whitlock modeling stress and anxiety in zebrafish jonathan m. To carry out the investigations, fish were divided into six groups six colour combinations and tested for a place preference in a conditioned place preference apparatus. Part of the neuromethods book series nm, volume 51. This methodology offers an incremental value to the drug addiction field, because it describes behavioral features associated to nicotineinduced cpp in zebrafish. Conditioned place preference behavior in zebrafish ncbi nih. Zebrafish place preference test maze engineers conduct science. Behavioral neuroscience of zebrafish methods of behavior. Behavioral quantification within the conditioned place preference cpp. Mazeengineers products are not designed for human consumption, testing, or clinical utilization. Pdf this protocol describes conditioned place preference cpp in zebrafish following a single exposure to a substance. I hope i can be forgiven for starting with my own book. The difference between anxiolytic and anxiogenic effects.
Behavioral screening for cocaine sensitivity in mutagenized zebrafish. The development of novel pharmacotherapies and targeted intervention strategies. The school nurse should identify this intervention as based on which principle of behavior therapy. The conditioned place preference assay is a form of classical conditioning box 2. Evaluation of drug reward the conditioned place preference cpp paradigm is used to evaluate the rewarding effects of a wide range of addictive drugs.
Ms was dissolved in system water and appropriate volumes of this stock. A preference test is an experiment in which animals are allowed free access to multiple environments which differ in one or more ways. In one they aggressively interacted with a mirror, the other chamber was lacking a mirror. Quantitative measure of cpp behavior final place preference baseline place. During training, a distinctive environmental cue a floor texture. We describe an interdisciplinary comparison of the effects of acute and chronic alcohol exposure in terms of their disturbance of light, dark and color preferences and the occurrence of parkinsonlike behavior in zebrafish through computer visual tracking, data mining, and behavioral and physiological analyses. Conditioned place preference behavior in zebrafish. Nida notes glossary national institute on drug abuse nida. Zebrafish and conditioned place preference the aquila digital.
Zebrafish neurobehavioral protocols book depository. We found that zebrafish in anxiolytic and anxious states, which are induced by. Our behavioral screen for cocaineinduced cpp in zebrafish is similar to that used with other species fig. Behavioral quantification within the conditioned place preference cpp paradigm serves as a measure of the rewarding qualities of a given substance. Books and links on fish behaviour how fish behave fish. An animal is placed inside a test chamber the conditioned stimulus and allowed access to an unconditioned stimulus a drug, food, odor, etc.
This protocol describes conditioned place preference cpp in zebrafish following a single exposure to a substance. Researchers repeatedly expose an animal to a drug in one chamber of a test cage, and subsequently place the animal in a foyer between that chamber and a second chamber that it. Conditioned place preference sylics contract research. Zebrafish preference was corroborated by behavioral analysis of several indicators. Conditioned place preference behavior in adult zebrafish. A kindergarten rule states that if unacceptable behavior occurs, a childs personalized fish will be moved to the sea grass.
Behavioral and molecular analysis of nicotineconditioned place. Just as in the rodent assay, zebrafish are placed into an environment with three chambers. The conditioned place preference cpp paradigm is a commonly used technique in behavioural neuroscience to evaluate rewarding and aversive effects of addictive drugs tzschentke, 2007, prus et al. Conditioned place preference an overview sciencedirect topics. A cpp setup consists of two compartments that differ in terms of tactile and visual properties, and a neutral middle compartment. Analysis of nicotineconditioned place preference in zebrafish. Tickle as the unconditioned stimulus and the effects of a consolidation interval. Behavioral characterization of nicotine biased place preference in zebrafish. Conditioned place preference olmstead 1994 maze engineers. Natural colour preference in the zebrafish danio rerio. By measuring the amount of time an animal spends in an area that has been associated with a stimulus, researchers can infer the animals liking for the stimulus. The role of midbrain dopamine in behaviors related to addiction has been exhaustedly researched 6, 7. Applied animal behaviour science newcastle university. Zebrafish conditioned place preference models of drug reinforcement and relapse to drug seeking.